Analysis of monoclonal antibodies against the membrane proximal external region (MPER) of the HIV-1 envelope protein. Application to immunogen design

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Analysis of monoclonal antibodies against the membrane proximal external region (MPER) of the HIV-1 envelope protein. Application to immunogen design

01/01/2015 - 31/12/2017

Principal Investigators: Julià Blanco

The membrane proximal external region (MPER) of the HIV envelope glycoprotein is a target for generating neutralizing antibodies. We have identified a miniprotein derived from gp41 which, in the form of proteoliposomes, generates strong humoral responses in murine models. Our results, however, demonstrate the presence of both neutralizing and non-neutralizing responses to the MPER. Therefore, in this project we propose:

1. To define the structural determinants associated with neutralizing and non-neutralizing activity.

2. To define the role of lipids in the structure of the protein.

3. To integrate the data in a new improved immunogen.

The methodology includes epitope mapping, antibody isolation and mutagenesis and molecular modelling and murine modelling of immunization.

  • Participant entities: IrsiCaixa AIDS Research Institute, Germans Trias i Pujol Institute for Health Science Research (IGTP), University of Vic-Central University of Catalonia (UVic-UCC)
  • Strategic line: Immunopathogenesis, Microbiome, Prevention, eradication and functional cure
  • Funding entity: Ministry of the Economy and Competitiveness - Carlos III Health Institute (ISCIII) - FEDER. PI14/01307

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Irsi Caixa

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'La Caixa' - Foundation Generalitat de Catalunya - Departament de Salut

 

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